Profauna Pet Probotics Frequently Asked Questions (FAQs)

What are Profauna Pet Probiotics?

Profauna 50 and Profauna 100 are among the highest count probiotics specifically developed to support your pet's healthy digestive tract.  The 8 strains of bacteria in Profauna’s proprietary powder mixture deliver 50-100 billion CFU per 1 gram scoop which provides both abundance and diversity of bacterial strains important for supporting the microbiome of your dog or cat.

What types of bacteria are used in Profauna Pet Probiotics and why?

The 8 strains of bacteria selected for Profauna were chosen for their performance in clinical trials and species-specific research, their ability to resist low pH (in the stomach), and their adherence properties. Making it all the way through the GI tract is important to support a pet’s gut health.  The 8 strains of bacteria (and all ingredients) in Profauna are sourced in the USA and include: Enterococcus thermophilus, Lactobacillus plantarum, Lactobacillus bulgaricus, Bifidobacteria animalis, Lactobacillus acidophilus, Lactobacillus brevis, Lactobacillus casei, and Bifidobacterium bifidum.

Are there any cautions, warnings or side-effects I should be aware of?

No. There are no known side-effects.

What’s the best way to give Profauna 100 or 50 to my pet?

Just add 1 scoop of powder to your pets wet or dry food.  That's it!  We recommend you put Profauna 100 on top of your pet's food to help protect the bacteria through the GI tract.

Can my pet take more than one scoop per day?

Yes.  Generally 1 scoop or 1 gram of powder is enough for your pet (often referred to as a dosage by pet parents).  However, different sized animals and the issue causing the problem you are trying to improve may require a higher daily intake than one scoop per day.  For example, if your dog has started to tolerate Profauna 100 (meaning his stools are looser again) you may need to increase the dose of Profauna 100.  It is best to consult with your veterinarian, but here is a table with suggested daily doses based on weight and taken from clinical trial data. 

Suggested daily feeding is 1 SCOOP PER DAY FOR ALL PETS AND WEIGHTS, but daily doses can be increased based on your consultation with your veterinarian to the following (based on animal weight):


grams per day

Profauna 50 or Profauna 100

CFU Amount

11 pounds or less

1 scoop daily (1 g) Profauna 50

50 billion

12 - 22 pounds

1-2 scoops daily (1-2 g) Profauna 50

50 - 100 billion

23-43 pounds

1-2 scoops daily (1-2 g) Profauna 100

100 – 200 billion

44-65 pounds

2-3 scoops daily (2-3 g)

200 – 300 billion

66-87 pounds

3-4 scoops daily (3-4 g)

300 – 400 billion

88 pounds and over

4-5 scoops daily (4-5 g)

400 – 500 billion

Directions: The dose should be given once daily, in the morning, or evening.  Add it directly onto the dog’s food. Profauna 100 may also be added to water and mixed in the dog’s food.

Is it necessary to Refrigerate Profauna Pet Probiotics?

No, it is not necessary to store Profauna Pet Probiotics in your refrigerator, but it can be safely stored in the fridge if you prefer. For best results, store at controlled room temperature (up to 77 °F) and always keep it out of the reach of children and animals.

Can my pet take Profauna Pet Probiotics with medications? 

There are currently no known drug interactions with Profauna 100.  However, if your pet is taking antibiotics, Profauna Pet Probiotics should be given in-between antibiotic doses thereby maximizing the probiotic effect. 

A recent study, led by Dr. Nassos Typas from the European Molecular Biology Laboratory in Heidelberg (Germany), has found that many non-antibiotic human-targeted drugs may have a direct effect on the gut microbiome composition.  This recent study suggests that several medications may affect the human microbiome, so we should be careful with our pet’s microbiome too. Seek advice from your veterinarian if your pet is taking certain non-antibiotic drugs such as anti-diabetic drugs like metformin and acarbose, proton pump inhibitors, non-steroidal anti-inflammatory drugs, and second-generation antipsychotics, opioids and statins. 

Here is the link:

What should I expect after I start my dog or cat on Profauna 100?

94% of dogs using Profauna 100 have an improvement in the quality and quantity of their stools within 30 days?  With any diet change, you can expect your dog to experience some stool changes, but in the end, probiotics are generally a safe way to improve the quality and quantity of your dog’s stool. 

With any diet, stress, or environment change, you can expect your cat to experience some stool changes, but in the end, probiotics are generally a safe way to improve the quality of your cat’s stool. 

Your pet should start seeing improvements in the first 1-2 weeks of introducing Profauna, but the most dramatic results should start to occur between 3-5 weeks. 

Will my pet taste Profauna Pet Probiotics on their food?

We’ve taken pride in creating a probiotic with essentially no flavor so your pet should not be able to detect it in the food.  In fact, 8 out of 10 pet owners who participated in our free trial program said their pets could not detect Profauna in their food. 

How long should my pet take Profauna Pet Probiotics?

Keeping your pet’s digestive system running optimally is essential to making sure that she/he stays healthy, active, and lives a full and healthy life. One way to support your pet’s digestive health is to give a daily probiotic supplement such as Profauna for life. Giving a probiotic long-term really depends on why you are using Profauna. If it is to boost the microbiome after antibiotics then short-term use is good. However, if your dog or cat has a chronic GI issue, consult with a veterinarian and ask them for advice on timing.

Where can I buy Profauna Pet Probiotics?

Profauna can be conveniently purchased right here at or you can also find it on 

How can I share my success story (or pet-timony) about Profauna 50 or Profauna 100?

We’d absolutely love to hear about your pet’s success story with Profauna.  Please email us at and don’t forget to send us some cute pictures. 

My shipment of Profauna Pet Probiotics was left outside all day by the shipper.  Is it still good to use?

We have conducted extreme temperature tests on Profauna 100 and we know that for up to 6 days in about 100 degrees there is no significant loss of bacterial count so it is good to use. However, depending on where you live and where the product was left you may want to open it and look at the powder. If the powder looks normal (off-white or creamy colored) with no black or brown bits in it, it is probably good to use. The bacteria tend to go brown once the freeze-drying has worn off and the bacteria is no longer alive. In general, the formula is hardy and should survive one small extreme of temperature. If you are concerned, please call us for a replacement.

Can Profauna 100 help my dog or cat with Chronic Diseases like IBD (inflammatory bowel disease)?

Profauna 100 was developed based on veterinarian feedback about human products they were using for IBD. The vets suggested a high count, multi-strain probiotic that would not require refrigeration and Profauna 100 was born! 

IBD in cats and dogs is characterized by chronic clinical signs such as vomiting, diarrhea, decreased appetite, and weight loss as well as histologic evidence of inflammation. Many of the treatments available to vets are human prescription drugs, but some newer, higher count probiotics can play a role.

  • Microbiota alterations in acute and chronic gastrointestinal inflammation of cats and dogs. Honneffer JB, Minamoto Y, Suchodolski JS. World J Gastroenterol 2014; 20(44): 16489-16497. Available from: URL:
  • Comparison of Microbiological, Histological, and Immunomodulatory Parameters in Response to Treatment with Either Combination Therapy with Prednisone and Metronidazole or a Multi-strain Probiotic in Dogs with Idiopathic Inflammatory Bowel Disease. Rossi G, Pengo G, Caldin M, Palumbo Piccionello A, Steiner JM, et al. (2014). PLoS ONE 9(4): e94699. doi:10.1371/journal.pone.0094699

Probiotics are live bacteria that have been shown (in dogs and cats) to modulate inflammatory and immune processes, and improve intestinal health; that really helps if your dog or cat has IBD.

There is also some evidence that shows certain dog breeds are at higher risk than other breeds for developing IBD, especially as they mature. IBD in cats is more common as they get older, senior cats are more at risk.

  • According to the American Kennel Club Canine Health Foundation ‘Several breeds may be at increased risk for developing IBD, including the German Shepherd dog, Boxer, Shar-Pei, Soft Coated Wheaton Terrier and Rottweiler.’ - See more at:
  • 2015 Aug;34:14-23. doi: 10.1016/j.anaerobe.2015.04.002. Epub 2015 Apr 8. Microbiota and probiotics in canine and feline welfare. Grześkowiak Ł et al. New challenges for probiotic applications include maintenance of obesity and overweight, urogenital tract infections, Helicobacter gastritis and parasitic infections. The probiotics of human origin appear to be among the new promising tools for the maintenance of pets' health. Therefore, more controlled trials are needed to characterize new and safe probiotic preparations with an impact on general health and well-being as well as health maintenance in dogs and cats.

It's important for your pet to consume probiotics in sufficient high quantities  because typically higher doses of live cells are optimal for specific ailments as well as for specific minor health problems. Profauna has at least 50 to 100 billion CFUs per serving, making it one of the highest count pet probiotics available on the market today and contains about 10x more than leading competitors. You can also check out some of our reviews from parents of dogs with IBD and read about their success by scrolling down under the product page

High count probiotics that work can be expensive, so at The Microbial LLC we have kept the costs of our product well below that of the human alternatives (our retail $35-$40 theirs over $60).

Here are some sources to support using probiotics in pets with IBD:

Vet Microbiol.2019 Apr;231:197-206. doi: 10.1016/j.vetmic.2019.03.020. Epub 2019 Mar 18. Influence of the intestinal microbiota on disease susceptibility in kittens with experimentally induced carriage of atypical enteropathogenic Escherichia coli. Watson VE et al. Enrichment of the intestinal microbiota with a commensal member of the feline mucosa-associated microbiota, Enterococcus hirae, ameliorated the effects of an EPEC experimental infection on intestinal function and water loss. 

Benef Microbes.2018 Jan 29;9(1):101-110. doi: 10.3920/BM2017.0023. Epub 2017 Oct 25. Effects of a probiotic on clinical and histologic variables and microbiota of cats with chronic constipation/megacolon: a pilot study. Rossi G et al. Treatment with probiotic in cats with chronic constipation and idiopathic megacolon showed significant clinical improvement after treatment, and histological parameters suggest a potential anti-inflammatory effect of SLAB51™, associated with a reduction of mucosal infiltration, and restoration of the number of interstitial cells of Cajal.

Top Companion Anim Med.2017 Sep;32(3):104-108. doi: 10.1053/j.tcam.2017.11.002. Epub 2017 Nov 28. Effect of Enterococcus faecium Strain SF68 on Gastrointestinal Signs and Fecal Microbiome in Cats Administered Amoxicillin-Clavulanate. Torres-Henderson C et al. The results show administering amoxicillin-clavulanate orally to cats commonly induces diarrhea and alters the gastrointestinal microbiome, and that feeding the probiotic SF68 can lessen some associated clinical abnormalities.

PLoS One. 2014 Apr 10;9(4):e94699. doi: 10.1371/journal.pone.0094699. Rossi G et al. Comparison of microbiological, histological, and immunomodulatory parameters in response to treatment with either combination therapy with prednisone and metronidazole or multi-strain probiotic in dogs with idiopathic inflammatory bowel disease. 

Pol_J_Vet_Sci 2011;14(1):165-71. Treatment of inflammatory bowel disease (IBD) in dogs and cats. Malewska K et al. ABSTRACT: The treatment of inflammatory bowel disease (IBD) possesses numerous difficulties owing to the unclear etiology of the disease. This article overviews the drugs used in the treatment of IBD depending on the intensity of clinical symptoms (Canine Inflammatory Bowel Disease Activity Index and Canine Chronic Enterophathy Clinical Activity Index). Patients demonstrating mild symptoms of the disease are usually placed on an appropriate diet which may be combined with immunomodulative or probiotic treatment. In moderate progression of IBD, 5-aminosalicylic acid (mesalazine or olsalazine) derivatives may be administered. Patients showing severe symptoms of the disease are usually treated with immunosuppressive drugs, antibiotics and elimination diet. Since the immune system plays an important role in the pathogenesis of the disease, the advancements in biological therapy research will contribute to the progress in the treatment of canine and feline IBD in the coming years.