PROFAUNA 100 Frequently Asked Questions (FAQs)


What is Profauna 100?

Profauna 100 is one of the highest count probiotics specifically developed to support a dog’s healthy digestive tract.  The 8 strains of bacteria in Profauna’s proprietary powder mixture deliver 100 billion CFU per 1 gram scoop which provides both abundance and diversity of bacterial strains important for supporting a dog’s microbiome.

What types of bacteria are used in Profauna 100 and why?

The 8 strains of bacteria selected for Profauna 100 were chosen for their performance in clinical trials and species-specific research, their ability to resist low pH (in the stomach), and their adherence properties. Making it all the way through the GI tract is important to support a dog’s gut health.  The 8 strains of bacteria in Profauna 100 are all sourced in the USA and include: Enterococcus thermophilus, Lactobacillus plantarum, Lactobacillus bulgaricus, Bifidobacteria animalis, Lactobacillus acidophilus, Lactobacillus brevis, Lactobacillus casei, and Bifidobacterium bifidum.

Are there any cautions or warnings I should be aware of?

No.  There are no known side effects.

What’s the best way to give Profauna 100 to my pet?

Just add 1 scoop of powder to your dog's wet or dry food.  That's it!  We recommend you put Profauna 100 on top of your dog’s food to help protect the bacteria through the GI tract.

Can my dog take more than one scoop per day?

Yes.  Generally 1 scoop or 1 gram of powder is enough for a dog (often referred to as a dosage by pet parents).  However, different breeds and the issue causing the problem you are trying to improve may require a higher daily intake than one scoop per day.  For example, if your dog has started to tolerate Profauna 100 (meaning his stools are looser again) you may need to increase the dose of Profauna.  It is best to consult with your veterinarian, but here is a table with suggested daily doses based on weight and taken from clinical trial data. 

Suggested daily feeding is 1 SCOOP PER DAY FOR ALL BREEDS AND WEIGHTS, but daily doses can be increased based on your consultation with your veterinarian to the following (based on animal weight):


mg per day

CFU Amount

22 pounds or less

1 scoop daily (1 g)

100 billion

23-43 pounds

1-2 scoops daily (1-2 g)

100 – 200 billion

44-65 pounds

2-3 scoops daily (2-3 g)

200 – 300 billion

66-87 pounds

3-4 scoops daily (3-4 g)

300 – 400 billion

88 pounds and over

4-5 scoops daily (4-5 g)

400 – 500 billion

Directions: The dose should be given once daily, in the morning, or evening.  Add it directly onto the dog’s food. Profauna 100 may also be added to water and mixed in the dog’s food.

Is it necessary to refrigerate Profauna 100 probiotics for my dog?

No, it is not necessary to store Profauna 100 in your refrigerator, but it can be safely stored in the refrigerator if you prefer.  For best results, store at controlled room temperature (up to 77 °F) and always keep it out of the reach of children and animals.

Can my dog take Profauna 100 with medications? 

There are currently no known drug dosage interactions with Profauna 100.  However, if your dog is taking antibiotics, Profauna 100 should be given in-between antibiotic doses thereby maximizing the probiotic effect. 

A recent study, led by Dr. Nassos Typas from the European Molecular Biology Laboratory in Heidelberg (Germany), has found that many non-antibiotic human-targeted drugs may have a direct effect on the gut microbiome composition.  This recent study suggests that several medications may affect the human microbiome, so we should be careful with our dog’s microbiome too. Seek advice from your veterinarian if your dog is taking certain non-antibiotic drugs such as anti-diabetic drugs like metformin and acarbose, proton pump inhibitors, non-steroidal anti-inflammatory drugs, and second-generation antipsychotics, opioids and statins. 

Here is the link:

What should I expect after I start my dog on Profauna 100?

Did you know 94% of dogs using Profauna 100 have an improvement in the quality and quantity of their stools within 30 days?  With any diet change, you can expect your dog to experience some stool changes, but in the end, probiotics are generally a safe way to improve the quality and quantity of your dog’s stool.  Your dog should start seeing improvements in the first 1-2 weeks of introducing Profauna 100, but the most dramatic results should start to occur between 3-5 weeks. 

Will my dog taste Profauna 100 on the dog food?

We’ve taken pride in creating a probiotic with essentially no flavor so your dog should not be able to detect it in the food.  In fact, 8 out of 10 pet owners who participated in our free trial program said their pets could not detect Profauna 100 in their food. 

How long should my dog take Profauna 100?

Keeping your dog’s digestive system running optimally is essential to making sure that he stays healthy, active, and lives a full and healthy life.  One way to support your dog’s digestive health is to give a daily probiotic supplement such as Profauna 100 for life. It depends on why you are using Profauna. If it is to boost the microbiome after antibiotics then short-term use is good. However, if your dog has a chronic GI issue, consult with a veterinarian and ask them for advice on timing.

Where can I buy Profauna 100?

Profauna 100 can be conveniently purchased right here at or you can visit our booth at a trade show near you. 

How can I share my success story (or pet-timony) about Profauna 100?

We’d absolutely love to hear about your pet’s success story with Profauna.  Please email us at and don’t forget to send us some cute pictures. 

Can I use Profauna 100 with my other non-canine pets (cat, cats)?

We have not tested Profauna 100 in any other pet, just dogs.

My shipment of Profauna 100 was left outside all day by the shipper.  Is it still good to use?

We have conducted extreme temperature tests on Profauna 100 and we know that for up to 6 days in about 100 degrees there is no significant loss of bacterial count so it is good to use. However, depending on where you live and where the product was left you may want to open it and look at the powder. If the powder looks normal (off-white or creamy colored) with no black or brown bits in it, it is probably good to use. The bacteria tend to go brown once the freeze-drying has worn off and the bacteria is no longer alive. In general, the formula is hardy and should survive one small extreme of temperature.

Can Profauna 100 help my dog with IBD (inflammatory bowel disease)?

Profauna 100 was developed based on veterinarian feedback about human products they were using for IBD. The vets suggested a high count, multi-strain probiotic and Profauna 100 was born! 

IBD in dogs is characterized by chronic clinical signs such as vomiting, diarrhea, decreased appetite, and weight loss as well as histologic evidence of inflammation. Many of the treatments available to vets are human prescription drugs, but some newer, higher count probiotics can play a role.

  • Microbiota alterations in acute and chronic gastrointestinal inflammation of cats and dogs. Honneffer JB, Minamoto Y, Suchodolski JS. World J Gastroenterol 2014; 20(44): 16489-16497. Available from: URL:
  • Comparison of Microbiological, Histological, and Immunomodulatory Parameters in Response to Treatment with Either Combination Therapy with Prednisone and Metronidazole or a Multi-strain Probiotic in Dogs with Idiopathic Inflammatory Bowel Disease. Rossi G, Pengo G, Caldin M, Palumbo Piccionello A, Steiner JM, et al. (2014). PLoS ONE 9(4): e94699. doi:10.1371/journal.pone.0094699

Probiotics are live bacteria that have been shown (in dogs and cats) to modulate inflammatory and immune processes, and improve intestinal health; that really helps if your dog has IBD. There is also some evidence that shows certain dog breeds are at higher risk than other breeds for developing IBD, especially as they mature.

It's important for your dog to consume probiotics in sufficient high quantities (or a high count probiotic) because typically higher doses of live cells are optimal for specific ailments or for specific minor health problems. Profauna 100 has 100 billion CFUs per serving, which is one of the highest count probiotics available on the market today and contains about 20x more than leading competitors. You can also check out some of our reviews from parents of dogs with IBD and read about their success by scroling down under the product page

High count probiotics that work can be expensive, so at The Microbial LLC we have kept the costs of our product well below that of the human alternatives (our retail $39.95 theirs over $60.00).

 Here are some sources to support using probiotics in dogs with IBD:

  • Malewska K1, Rychlik A, Nieradka R, Kander M. Treatment of inflammatory bowel disease (IBD) in dogs and cats. Pol J Vet Sci. 2011;14(1):165-71. – you can keep this but my refs are more recent.
  • Rossi G1, Pengo G2, Caldin M3, Palumbo Piccionello A1, Steiner JM4, Cohen ND5, Jergens AE6, Suchodolski JS4. Comparison of microbiological, histological, and immunomodulatory parameters in response to treatment with either combination therapy with prednisone and metronidazole or probiotic VSL#3 strains in dogs with idiopathic inflammatory bowel disease. PLoS One. 2014 Apr 10;9(4):e94699. doi: 10.1371/journal.pone.0094699. eCollection 2014.